Motif ID: CCAGCAU

Z-value: 1.340


Mature miRNA associated with seed CCAGCAU:

NamemiRBase Accession
hsa-miR-338-3p MIMAT0000763



Activity profile for motif CCAGCAU.

activity profile for motif CCAGCAU


Sorted Z-values histogram for motif CCAGCAU

Sorted Z-values for motif CCAGCAU



Network of associatons between targets according to the STRING database.



First level regulatory network of CCAGCAU

PNG image of the network

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Top targets:


Promoter Score Refseq Gene Symbol Gene Name
chr12_-_54982271 4.195 NM_006741
PPP1R1A
protein phosphatase 1, regulatory (inhibitor) subunit 1A
chr3_-_134093235 4.138 NM_016201
AMOTL2
angiomotin like 2
chr11_+_130318457 4.057 NM_139055
ADAMTS15
ADAM metallopeptidase with thrombospondin type 1 motif, 15
chr20_+_45338125 4.027 NM_030777
SLC2A10
solute carrier family 2 (facilitated glucose transporter), member 10
chr3_+_85008031 4.019 NM_001167674
NM_001167675
CADM2

cell adhesion molecule 2

chr16_-_51184507 3.827 NM_001127892
SALL1
sal-like 1 (Drosophila)
chr9_+_124329398 3.806 NM_032552
DAB2IP
DAB2 interacting protein
chr16_-_51185150 3.675 NM_002968
SALL1
sal-like 1 (Drosophila)
chr3_+_238274 3.247 NM_001253387
NM_006614
CHL1

cell adhesion molecule with homology to L1CAM (close homolog of L1)

chr22_-_44258210 3.175 NM_014351
SULT4A1
sulfotransferase family 4A, member 1
chr15_+_85359910 3.167 NM_020778
ALPK3
alpha-kinase 3
chr10_+_102106771 3.164 NM_005063
SCD
stearoyl-CoA desaturase (delta-9-desaturase)
chr7_+_116166346 3.094 NM_001172896
NM_001172897
CAV1

caveolin 1, caveolae protein, 22kDa

chr10_-_123357503 2.941 NM_000141
NM_001144917
NM_001144918
NM_001144919
NM_022970
FGFR2




fibroblast growth factor receptor 2




chr15_+_73976621 2.880 NM_001024736
NM_025240
CD276

CD276 molecule

chr3_+_85775631 2.846 NM_153184
CADM2
cell adhesion molecule 2
chr8_-_18871163 2.793 NM_015310
PSD3
pleckstrin and Sec7 domain containing 3
chr15_+_62939509 2.672 NM_015059
TLN2
talin 2
chr7_+_116164838 2.603 NM_001753
CAV1
caveolin 1, caveolae protein, 22kDa
chr17_-_48278987 2.570 NM_000088
COL1A1
collagen, type I, alpha 1
chr5_+_140723600 2.566 NM_018916
NM_032011
PCDHGA3

protocadherin gamma subfamily A, 3

chr10_-_123356158 2.530 NM_001144915
FGFR2
fibroblast growth factor receptor 2
chr21_-_42879908 2.527 NM_001135099
TMPRSS2
transmembrane protease, serine 2
chr5_+_140718353 2.523 NM_018915
NM_032009
PCDHGA2

protocadherin gamma subfamily A, 2

chr7_+_116165043 2.496 NM_001172895
CAV1
caveolin 1, caveolae protein, 22kDa
chr5_+_140753480 2.482 NM_018919
NM_032086
PCDHGA6

protocadherin gamma subfamily A, 6

chr10_+_28966419 2.456 NM_012342
BAMBI
BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
chr8_-_17658385 2.448 NM_001001924
NM_001001925
MTUS1

microtubule associated tumor suppressor 1

chr5_+_140782367 2.369 NM_018921
NM_032089
PCDHGA9

protocadherin gamma subfamily A, 9

chr11_+_100557851 2.312 NM_152432
ARHGAP42
Rho GTPase activating protein 42
chr10_-_123353771 2.311 NM_001144916
FGFR2
fibroblast growth factor receptor 2
chr5_+_140739589 2.251 NM_018923
NM_032096
PCDHGB2

protocadherin gamma subfamily B, 2

chr8_-_18666295 2.224 NM_206909
PSD3
pleckstrin and Sec7 domain containing 3
chr5_+_140864626 2.214 NM_018928
NM_032406
PCDHGC4

protocadherin gamma subfamily C, 4

chr2_+_120189342 2.123 NM_183240
TMEM37
transmembrane protein 37
chr1_+_95558072 2.093 NM_001199679
TMEM56
transmembrane protein 56
chr8_-_17580024 2.092 NM_001166393
MTUS1
microtubule associated tumor suppressor 1
chrX_-_139866582 2.055 NM_004065
CDR1
cerebellar degeneration-related protein 1, 34kDa
chr16_+_46918216 1.982 NM_133443
GPT2
glutamic pyruvate transaminase (alanine aminotransferase) 2
chr12_-_113573267 1.976 NM_001193521
RASAL1
RAS protein activator like 1 (GAP1 like)
chr2_-_100939194 1.969 NM_198461
LONRF2
LON peptidase N-terminal domain and ring finger 2
chr21_-_42880080 1.931 NM_005656
TMPRSS2
transmembrane protease, serine 2
chr8_-_17579729 1.928 NM_001001931
MTUS1
microtubule associated tumor suppressor 1
chr11_-_75379414 1.918 NM_033063
NM_207577
MAP6

microtubule-associated protein 6

chr8_-_143858436 1.905 NM_177476
NM_177477
LYNX1

Ly6/neurotoxin 1

chr5_+_140729723 1.904 NM_018922
NM_032095
PCDHGB1

protocadherin gamma subfamily B, 1

chr13_+_51796363 1.897 NM_001242312
NM_145019
FAM124A

family with sequence similarity 124A

chr8_-_17555158 1.892 NM_020749
MTUS1
microtubule associated tumor suppressor 1
chr10_-_123353330 1.891 NM_001144913
NM_001144914
FGFR2

fibroblast growth factor receptor 2

chr5_+_140749830 1.891 NM_018924
NM_032097
PCDHGB3

protocadherin gamma subfamily B, 3

chr12_+_31079837 1.887 NM_001080509
TSPAN11
tetraspanin 11
chr17_+_43971655 1.886 NM_001123066
NM_001123067
NM_001203251
NM_001203252
NM_005910
NM_016834
NM_016835
NM_016841
MAPT







microtubule-associated protein tau







chr1_+_95582827 1.877 NM_152487
TMEM56
transmembrane protein 56
chr4_+_154074269 1.873 NM_001130067
TRIM2
tripartite motif containing 2
chr11_-_123525300 1.850 NM_001040151
NM_018400
SCN3B

sodium channel, voltage-gated, type III, beta

chr12_-_48152180 1.843 NM_001098532
NM_006105
RAPGEF3

Rap guanine nucleotide exchange factor (GEF) 3

chr5_+_140810126 1.832 NM_003735
NM_032094
PCDHGA12

protocadherin gamma subfamily A, 12

chr11_-_17410205 1.801 NM_000525
KCNJ11
potassium inwardly-rectifying channel, subfamily J, member 11
chr16_+_46919101 1.797 NM_001142466
GPT2
glutamic pyruvate transaminase (alanine aminotransferase) 2
chr1_+_171154387 1.789 NM_001460
FMO2
flavin containing monooxygenase 2 (non-functional)
chr5_-_149669400 1.763 NM_015981
NM_171825
CAMK2A

calcium/calmodulin-dependent protein kinase II alpha

chr5_+_140855543 1.753 NM_002588
NM_032402
NM_032403
PCDHGC3


protocadherin gamma subfamily C, 3


chr4_-_100242487 1.749 NM_000668
ADH1A
ADH1B
alcohol dehydrogenase 1A (class I), alpha polypeptide
alcohol dehydrogenase 1B (class I), beta polypeptide
chr5_+_63802427 1.744 NM_001029875
RGS7BP
regulator of G-protein signaling 7 binding protein
chr5_+_140868721 1.695 NM_018929
NM_032407
PCDHGC5

protocadherin gamma subfamily C, 5

chr2_-_217560247 1.695 NM_000599
IGFBP5
insulin-like growth factor binding protein 5
chr20_-_41818464 1.688 NM_007050
NM_133170
PTPRT

protein tyrosine phosphatase, receptor type, T

chr5_+_140800536 1.670 NM_018914
NM_032091
NM_032092
PCDHGA11


protocadherin gamma subfamily A, 11


chr15_-_90222572 1.654 NM_001145311
NM_002666
PLIN1

perilipin 1

chr16_-_77468930 1.653 NM_199355
ADAMTS18
ADAM metallopeptidase with thrombospondin type 1 motif, 18
chr20_-_48184652 1.651 NM_000961
PTGIS
prostaglandin I2 (prostacyclin) synthase
chr11_+_120195837 1.649 NM_001198670
NM_001198671
NM_001198672
NM_001198673
NM_001198674
NM_001198675
NM_174926
TMEM136






transmembrane protein 136






chrX_+_2746810 1.645 NM_001079855
NM_001184702
NM_001184703
NM_001184704
NM_003918
GYG2




glycogenin 2




chr16_-_10674443 1.633 NM_001424
EMP2
epithelial membrane protein 2
chr12_-_102872329 1.613 NM_001111284
IGF1
insulin-like growth factor 1 (somatomedin C)
chr5_+_140743755 1.606 NM_018918
NM_032054
PCDHGA5

protocadherin gamma subfamily A, 5

chr17_+_47653176 1.598 NM_007225
NXPH3
neurexophilin 3
chr1_+_161228516 1.588 NM_001102566
PCP4L1
Purkinje cell protein 4 like 1
chr4_+_148402068 1.586 NM_001166055
NM_001957
EDNRA

endothelin receptor type A

chr5_+_149997205 1.579 NM_001166209
SYNPO
synaptopodin
chr10_-_99393175 1.523 NM_178832
MORN4
MORN repeat containing 4
chr7_+_45614081 1.515 NM_021116
ADCY1
adenylate cyclase 1 (brain)
chr9_-_91793681 1.506 NM_016848
SHC3
SHC (Src homology 2 domain containing) transforming protein 3
chr11_-_17410863 1.503 NM_001166290
KCNJ11
potassium inwardly-rectifying channel, subfamily J, member 11
chr5_+_140797243 1.496 NM_018927
PCDHGB7
protocadherin gamma subfamily B, 7
chr7_-_11871735 1.480 NM_015204
THSD7A
thrombospondin, type I, domain containing 7A
chr8_+_30241943 1.474 NM_001008710
NM_001008711
NM_001008712
NM_006867
RBPMS



RNA binding protein with multiple splicing



chr2_+_241375030 1.473 NM_002081
GPC1
glypican 1
chr11_+_69455837 1.466 NM_053056
CCND1
cyclin D1
chr10_-_90967048 1.449 NM_003956
CH25H
cholesterol 25-hydroxylase
chr20_-_50384899 1.446 NM_006045
ATP9A
ATPase, class II, type 9A
chr5_-_125930604 1.445 NM_001182
NM_001201377
NM_001202404
ALDH7A1


aldehyde dehydrogenase 7 family, member A1


chr2_-_56151273 1.434 NM_001039348
NM_001039349
EFEMP1

EGF containing fibulin-like extracellular matrix protein 1

chr14_+_38677186 1.430 NM_001049
SSTR1
somatostatin receptor 1
chr5_+_140792507 1.426 NM_018913
NM_032090
PCDHGA10

protocadherin gamma subfamily A, 10

chr11_+_124789145 1.407 NM_001037558
HEPN1
hepatocellular carcinoma, down-regulated 1
chr17_+_36584692 1.399 NM_001199417
ARHGAP23
Rho GTPase activating protein 23
chr15_+_71433787 1.394 NM_024817
THSD4
thrombospondin, type I, domain containing 4
chr10_-_97321094 1.386 NM_015385
NM_024991
SORBS1

sorbin and SH3 domain containing 1

chr22_+_35937351 1.383 NM_014310
RASD2
RASD family, member 2
chr12_-_113574020 1.379 NM_001193520
NM_004658
RASAL1

RAS protein activator like 1 (GAP1 like)

chr6_+_151646634 1.376 NM_144497
AKAP12
A kinase (PRKA) anchor protein 12
chr5_+_140710203 1.375 NM_018912
NM_031993
PCDHGA1

protocadherin gamma subfamily A, 1

chr4_+_154125589 1.363 NM_015271
TRIM2
tripartite motif containing 2
chr2_+_201450730 1.358 NM_001159
AOX1
aldehyde oxidase 1
chr10_-_97200895 1.357 NM_001034954
NM_001034955
NM_001034956
NM_001034957
NM_006434
SORBS1




sorbin and SH3 domain containing 1




chr5_+_140767402 1.353 NM_003736
NM_032098
PCDHGB4

protocadherin gamma subfamily B, 4

chr5_+_172068188 1.345 NM_001142651
NEURL1B
neuralized homolog 1B (Drosophila)
chr6_-_138893666 1.342 NM_020464
NHSL1
NHS-like 1
chr3_+_14860468 1.327 NM_152536
FGD5
FYVE, RhoGEF and PH domain containing 5
chr6_-_99797530 1.327 NM_032511
C6orf168
chromosome 6 open reading frame 168
chr12_-_48152860 1.327 NM_001098531
RAPGEF3
Rap guanine nucleotide exchange factor (GEF) 3
chr10_-_99393851 1.322 NM_001098831
MORN4
MORN repeat containing 4
chr11_+_74862031 1.318 NM_001145212
NM_007256
SLCO2B1

solute carrier organic anion transporter family, member 2B1

chr1_-_202612580 1.318 NM_001136504
SYT2
synaptotagmin II
chr12_-_16760935 1.317 NM_001001395
NM_001243609
LMO3

LIM domain only 3 (rhombotin-like 2)

chr6_+_151561094 1.309 NM_005100
AKAP12
A kinase (PRKA) anchor protein 12
chr14_+_24837225 1.305 NM_001198965
NM_004554
NFATC4

nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4

chr4_-_152147520 1.303 NM_001009555
NM_001128923
SH3D19

SH3 domain containing 19

chr1_-_31381436 1.301 NM_014654
SDC3
syndecan 3
chr4_+_77356252 1.301 NM_020859
SHROOM3
shroom family member 3
chr4_-_152096655 1.298 NM_001128924
SH3D19
SH3 domain containing 19
chr1_-_68299047 1.294 NM_018841
GNG12
guanine nucleotide binding protein (G protein), gamma 12
chr11_-_76381787 1.293 NM_001128922
LRRC32
leucine rich repeat containing 32
chr10_-_118501981 1.261 NM_025015
HSPA12A
heat shock 70kDa protein 12A
chr13_+_113623496 1.256 NM_001112732
MCF2L
MCF.2 cell line derived transforming sequence-like
chr5_+_140777603 1.254 NM_018925
NM_032099
PCDHGB5

protocadherin gamma subfamily B, 5

chr2_-_20424627 1.251 NM_002997
SDC1
syndecan 1
chr1_+_159141376 1.248 NM_021189
NM_001127173
CADM3

cell adhesion molecule 3

chr9_+_101705710 1.230 NM_001855
COL15A1
collagen, type XV, alpha 1
chr8_+_17396285 1.230 NM_001164771
NM_003046
SLC7A2

solute carrier family 7 (cationic amino acid transporter, y+ system), member 2

chr2_-_183731279 1.226 NM_001463
FRZB
frizzled-related protein
chr6_+_150690027 1.225 NM_001164694
NM_001164695
NM_203395
IYD


iodotyrosine deiodinase


chr7_-_131241321 1.218 NM_001018111
NM_005397
PODXL

podocalyxin-like

chr5_+_34656367 1.217 NM_001145522
NM_015577
RAI14

retinoic acid induced 14

chr12_-_102874373 1.215 NM_000618
NM_001111283
NM_001111285
IGF1


insulin-like growth factor 1 (somatomedin C)


chr1_-_160039946 1.210 NM_002241
KCNJ10
potassium inwardly-rectifying channel, subfamily J, member 10
chr6_-_138820578 1.209 NM_001144060
NHSL1
NHS-like 1
chr5_+_140734767 1.201 NM_018917
NM_032053
PCDHGA4

protocadherin gamma subfamily A, 4

chr16_+_66400524 1.200 NM_001795
CDH5
cadherin 5, type 2 (vascular endothelium)
chr16_-_75528925 1.199 NM_021615
CHST6
carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 6
chr12_+_50497790 1.199 NM_005276
GPD1
glycerol-3-phosphate dehydrogenase 1 (soluble)
chr3_+_12045803 1.197 NM_003178
NM_133625
SYN2

synapsin II

chr19_-_460995 1.186 NM_012435
SHC2
SHC (Src homology 2 domain containing) transforming protein 2
chr5_+_140787769 1.179 NM_018926
NM_032100
PCDHGB6

protocadherin gamma subfamily B, 6

chr8_+_144295067 1.177 NM_178172
GPIHBP1
glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1
chr6_+_132129154 1.169 NM_006208
ENPP1
ectonucleotide pyrophosphatase/phosphodiesterase 1
chr5_+_34656595 1.167 NM_001145520
RAI14
retinoic acid induced 14
chr7_-_51384458 1.164 NM_015198
COBL
cordon-bleu homolog (mouse)
chr4_-_139163222 1.161 NM_014331
SLC7A11
solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
chr10_-_45474210 1.158 NM_007021
C10orf10
chromosome 10 open reading frame 10
chr20_-_48099178 1.148 NM_004975
KCNB1
potassium voltage-gated channel, Shab-related subfamily, member 1
chr3_+_49591897 1.140 NM_003458
BSN
bassoon (presynaptic cytomatrix protein)
chr10_-_50323419 1.139 NM_001031746
NM_144984
VSTM4

V-set and transmembrane domain containing 4

chr6_-_105584982 1.138 NM_001199563
BVES
blood vessel epicardial substance
chr10_+_128593994 1.136 NM_001380
DOCK1
dedicator of cytokinesis 1
chr1_-_203320287 1.129 NM_002023
FMOD
fibromodulin
chr19_+_54466289 1.126 NM_031895
CACNG8
calcium channel, voltage-dependent, gamma subunit 8
chr7_+_30960914 1.123 NM_001185060
NM_001185061
NM_001185062
AQP1


aquaporin 1 (Colton blood group)


chr3_+_61547179 1.115 NM_002841
PTPRG
protein tyrosine phosphatase, receptor type, G
chr1_+_61542928 1.114 NM_001145511
NFIA
nuclear factor I/A
chr1_-_156647088 1.111 NM_006617
NES
nestin
chr19_-_49258646 1.109 NM_000148
FUT1
fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, H blood group)
chr11_+_74870824 1.105 NM_001145211
SLCO2B1
solute carrier organic anion transporter family, member 2B1
chr11_-_35441501 1.104 NM_001195728
NM_001252652
SLC1A2

solute carrier family 1 (glial high affinity glutamate transporter), member 2

chrX_-_10588458 1.104 NM_000381
NM_033289
MID1

midline 1 (Opitz/BBB syndrome)

chr10_-_118031542 1.098 NM_145793
GFRA1
GDNF family receptor alpha 1
chr12_+_12938540 1.094 NM_030817
APOLD1
apolipoprotein L domain containing 1
chr16_+_20420854 1.094 NM_017888
ACSM5
acyl-CoA synthetase medium-chain family member 5
chr11_-_79151694 1.089 NM_001098816
ODZ4
odz, odd Oz/ten-m homolog 4 (Drosophila)
chr2_+_46524448 1.077 NM_001430
EPAS1
endothelial PAS domain protein 1
chr3_-_57199402 1.076 NM_017563
IL17RD
interleukin 17 receptor D
chr18_-_48346297 1.067 NM_001127175
NM_001127176
NM_031939
MRO


maestro


chr11_-_33744497 1.063 NM_001127227
CD59
CD59 molecule, complement regulatory protein
chr14_+_63671101 1.062 NM_020663
RHOJ
ras homolog gene family, member J
chr5_+_180467224 1.057 NM_152547
BTNL9
butyrophilin-like 9
chr1_-_21995793 1.056 NM_001145657
NM_002885
RAP1GAP

RAP1 GTPase activating protein

chr14_+_24838301 1.052 NM_001198966
NFATC4
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
chr22_-_44708730 1.050 NM_001099294
KIAA1644
KIAA1644
chr14_-_27066959 1.047 NM_002515
NM_006489
NM_006491
NOVA1


neuro-oncological ventral antigen 1


chr7_+_30951414 1.045 NM_198098
AQP1
aquaporin 1 (Colton blood group)
chr15_+_41851153 1.045 NM_006293
TYRO3
TYRO3 protein tyrosine kinase
chr11_-_16430425 1.035 NM_001145811
SOX6
SRY (sex determining region Y)-box 6
chr1_+_204797774 1.034 NM_001005388
NM_001005389
NM_001160332
NM_001160333
NM_015090
NFASC




neurofascin




chr12_-_16759531 1.032 NM_018640
LMO3
LIM domain only 3 (rhombotin-like 2)
chr3_-_149375584 1.031 NM_001168280
NM_015472
WWTR1

WW domain containing transcription regulator 1

chr15_+_51633712 1.025 NM_181789
GLDN
gliomedin
chr1_-_163172624 1.023 NM_001195303
RGS5
regulator of G-protein signaling 5
chr2_+_101436491 1.018 NM_002518
NPAS2
neuronal PAS domain protein 2
chr14_+_24836144 1.013 NM_001136022
NM_001198967
NFATC4

nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4

chr11_-_33744272 1.009 NM_001127223
NM_001127225
NM_001127226
CD59


CD59 molecule, complement regulatory protein


chrX_-_10544852 1.007 NM_001193277
MID1
midline 1 (Opitz/BBB syndrome)
chr1_+_16091458 1.005 NM_001024216
FBLIM1
filamin binding LIM protein 1
chr11_+_118478305 1.003 NM_001144758
NM_001144759
PHLDB1

pleckstrin homology-like domain, family B, member 1

chr22_-_36424397 0.999 NM_001082578
NM_001082579
RBFOX2

RNA binding protein, fox-1 homolog (C. elegans) 2

chr18_+_47088400 0.996 NM_006033
LIPG
lipase, endothelial
chr1_-_163172863 0.996 NM_003617
RGS5
regulator of G-protein signaling 5
chr8_-_143859638 0.994 NM_023946
NM_177457
LYNX1

Ly6/neurotoxin 1

chr9_+_140033588 0.993 NM_000832
NM_001185090
NM_001185091
NM_007327
NM_021569
GRIN1




glutamate receptor, ionotropic, N-methyl D-aspartate 1




chr2_+_26915390 0.993 NM_002246
KCNK3
potassium channel, subfamily K, member 3

Gene Ontology Analysis

Gene overrepresentation in process category:

Enrichment   P-value GO Accession GO Term
19.64 1.29e-20 GO:0007156 homophilic cell adhesion
9.63 3.92e-14 GO:0016337 cell-cell adhesion
5.08 4.82e-11 GO:0007155 cell adhesion
5.08 4.82e-11 GO:0022610 biological adhesion
7.99 1.70e-03 GO:0030111 regulation of Wnt receptor signaling pathway
9.33 8.86e-03 GO:0060828 regulation of canonical Wnt receptor signaling pathway
1.26 1.07e-02 GO:0009987 cellular process
11.19 1.36e-02 GO:0008360 regulation of cell shape
13.32 3.05e-02 GO:0030177 positive regulation of Wnt receptor signaling pathway

Gene overrepresentation in compartment category:

Enrichment   P-value GO Accession GO Term
2.47 3.47e-17 GO:0071944 cell periphery
2.48 5.92e-17 GO:0005886 plasma membrane
1.66 8.23e-10 GO:0016020 membrane
1.70 1.24e-07 GO:0044425 membrane part
1.76 7.81e-07 GO:0031224 intrinsic to membrane
1.73 5.25e-06 GO:0016021 integral to membrane
2.09 9.40e-04 GO:0044459 plasma membrane part
3.16 6.83e-03 GO:0030054 cell junction

Gene overrepresentation in function category:

Enrichment   P-value GO Accession GO Term
4.10 1.58e-06 GO:0005509 calcium ion binding
15.98 1.93e-02 GO:0005158 insulin receptor binding
74.56 4.29e-02 GO:0015272 ATP-activated inward rectifier potassium channel activity